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Enzymes Aim To investigate how the substrate concentration (H2O2) affects the activity of catalase on hydrogen peroxide. Introduction Enzymes are protein molecules, which can be defined as biological catalysts. A catalyst is a molecule that speeds up a chemical reaction, but remains unchanged at the end of the reaction. An enzyme catalyses virtually every metabolic reaction, which takes place within a living organism. Enzymes have a globular protein structure. The enzyme molecules are coiled into a precise three-dimensional shape, with hydrophillic R groups (side chains) on the outside of the molecule ensuring that they are soluble. Enzyme molecules also have an active site, which is usually a cleft of depression, to which another molecule or molecules can bind. This molecule is the substrate of the enzyme. The shape of the active site allows the substrate to fit perfectly, and to be held in place by temporary bonds, which form between the substrate and some of...

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