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Introduction In this experiment we will be experimenting to find out the effect of enzyme concentration on reaction rates. Enzymes are proteins produced by living cells; they act as catalysts in biochemical reactions. A catalyst affects the rate of a chemical reaction. One benefit of enzyme catalysis is that the cell can carry out complex chemical activities at a relatively low temperature. In biochemical reactions, the enzyme, E, combines reversibly with its specific substrate, S, to form a complex, ES. One result of this temporary union is a reduction in the energy required to activate the reaction of the substrate molecule so that P, the products of the reaction, are formed. In summary: E+S=ES=E+P Note that the enzyme is not changed in the reaction and can recycle to break down additional substrate molecules. Each enzyme is specific for a particular reaction. In practice, this specificity permits one to mix a purified substrate...

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