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"AN INVESTIGATION TO FIND OUT THE OPTIMUM TEMPERATURE FOR THE ACTIVITY OF LIPASE" * AIM To investigate the effect of temperature has on enzyme activity and to investigate the hypothesis that there is an optimum temperature for the activity of the enzyme lipase. * INTRODUCTION Enzymes are biological catalysts made up of protein molecules, usually with a globular structure. They accelerate chemical reactions in organisms that would otherwise occur to slow. (Green, Stout, Taylor, 1991) An enzyme that breaks down larger molecules into smaller ones is known as a breaker enzyme and involves a catabolic reaction. Where as builder enzymes build large molecules from smaller ones, and involve an anabolic reaction Lipase involves a catabolic reaction and is a digestive pancreatic enzyme which works to hydrolyses fats into glycerol and fatty acids. (Simpkins, Williams, 1987) These products will react with the Sodium Carbonate (put into the mixture to raise the pH) and in effect lower...

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