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What effect does the pH have on the enzyme diastase? What effect does the temperature have on the activity of the enzyme diastase?
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Problem: * What effect does the pH have on the enzyme diastase? * What effect does the temperature have on the activity of the enzyme diastase? Hypothesis: * The optimum pH for the diastase enzyme to work will be at 37oC. The temperature of 10oC and 20oC are going to show that they are too low for the enzyme to function while the temperature of 70oC will show that it is a too high for the enzyme to function in. * The optimum pH for the diastase enzyme will be 7. The pH of 1 and 3 will be too acidic while the pH of 10 will be alkaline for the enzyme. Purpose: * To determine the effect of pH and temperature on the enzyme activity Materials: * 1% starch solution * Lugol's iodine * Buffer solution * 0.2% diastase * 4 test tubes * 2 hot plates * Ice * 5 dropper pipettes * Spot plate * Thermometer Procedure: A 1. The 4 test tubes have...
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