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What do you think will be the Relationship between the Concentration of Protease and the Rate of Breakdown of the Protein?
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What do you think will be the Relationship between the Concentration of Protease and the Rate of Breakdown of the Protein? Hypothesis The higher the concentration of alkali Trypsin solution, the faster the breakdown of the protein casein. Null Hypothesis The less concentration of alkali Trypsin solution, the faster the breakdown of the protein casein. Variables Independent Variable * Enzyme concentration Dependent Variable * The absorption of light using a calorimeter. Controlled Variables * Temperature of the water bath °C * pH of enzyme. * Volume of Milk Powder. Apparatus * 25cm3 Milk powder - 5ml of milk powder (Casein) within each test tube. * Standard Alkali Trypsin Solution * 6 Test Tubes * Test Tube Rack * 2 Measuring Pipettes * Calorimeter * Stopwatch * Water Bath * Thermometer * Distilled Water * Buffer Method 1. In preparation to start the experiment make sure that the water bath is at 30°C. Having the water warm creates heat energy, which makes the substrate and the enzyme move faster, creating more collisions between...
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