Logged out
Access All our Essays in an Instant
Swap your work for FREE access, or pay £4.99 for instant access
Use of a redox indicator to show dehydrogenase activity.
Member rating: No Rating | Words: | Submitted: Tue May 04 2004
Page Preview
On the left is an image preview of every page of this document, and below are the first 150 words with formatting removed:
USE OF A REDOX INDICATOR TO SHOW DEHYDROGENASE ACTIVITY INTRODUCTION The purpose of this experiment is to investigate the effect of temperature on the activity of dehydrogenase and for this experiment; the source of dehydrogenise is a respiring yeast cell. For this experiment, Triphenyl Tetrazolium Chloride (TTC) will be used as the redox indicator. TTC is colourless when oxidized but forms red insoluble compounds called formazans when reduced. Reduced/colourless TTC TTC Oxidized/red TTC will be used in this experiment to show the presence of active dehydrogenase enzyme as this is indicated by a change in colour. APPARATUS * Respiring yeast suspension * Triphenyl Tetrazolium Chloride solution * Distilled water * Test tubes * Graduated pipettes or syringes * Glass rods * Water Baths * Thermometer * Stopwatch KEY VARIABLES Independent Variable = Respiring Yeast Dependent Variable = enzyme activity Controlled Variables= time and temperature RISK ASSESMENT * Triphenyl Tetrazolium Chloride is harmful * Wear a lab coat to protect skin and clothes incase of spillage. * Wear eye goggles as enzymes irritate the eyes. * Mop...
Get instant access
- Instant, unlimited access to our documents in full
- Swap your work for free access, or pay £4.99
- To see the full version of this document and 146,186 others
Receive email updates for this category
- Simply tell us your email address and receive a weekly Study Help Email for FREE
- Receive 3 FREE essay views with each email
- Get all the latest essays from Coursework.Info & discussion from TheStudentRoom.co.uk
