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To investigate the effect of temperature on the enzyme catalase.
Member rating: No Rating | Words: | Submitted: Wed Oct 08 2003
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To investigate the effect of temperature on the enzyme catalase Aim: To investigate the effect of temperature on the enzyme catalase Apparatus Celery extract (catalase) Labels 20 Volume hydrogen peroxide Distilled water 6 100cm beakers 20cm and 50 cm measuring cylinders Balance Stopwatch Trough Clamps & stand 150cm conical flask + bung + tubing + 3 way tap 10cm syringe Beehive shelf Gas syringe Goggles Water bath at 25 & 35°C 250cm beakers & thermometer for water bath Method 1.Weigh out 1 sample of celery extract of 10g in a conical flask. 2.Place in a water bath at 25°C. 3.Place about 20cm hydrogen peroxide solution in a container in the same water bath. 4.Leave for 10 min to equilibrate. 5.Set up the apparatus as follows: -Fill a trough with water -Fill a measuring cylinder with water and invert in the trough (ensure that no air bubbles are in the water) -Secure the cylinder with a clamp & stand -Position the bung and 3 ways tap to fit on a conical flask, ensure that this can be held in...
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