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To investigate the effect of pH on the activity of the enzyme Trypsin
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| Submitted: Thu Jul 11 2002
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To investigate the effect of pH on the activity of the enzyme Trypsin Aim To investigate the effect of pH on the activity of the enzyme Trypsin using a range of buffer solutions and exposed photographic negative. Hypothesis Trypsin will have an optimum pH (the rate of reaction will be highest at that pH) and the rate of reaction will decrease below and above the optimum pH. Apparatus 3% trypsin solution Buffer solutions at pHs 3,5,7,9 and 10 5 pieces of exposed photographic negative (1.5cm2) 5 pieces of wire (about 15cm long) 5 test tubes 2 10cm3 graduated pipettes 2 Pipette fillers 1 thermostatically controlled water bath 1 thermometer 1 stop clock Method The 5 pieces of exposed photographic negative were put onto the ends of the 5 pieces of wire. 5cm3 of trypsin solution was put in to a test tube. 2cm3 of buffer solution at pH 7 was added to the test tube. The test tube was put into the water bath (set at 25?C) and...
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