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To investigate the effect of enzyme concentration on rate of reaction.  

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PLANNING AIM: To investigate the effect of enzyme concentration on rate of reaction. To determine the effects of this analogy, catalase the enzyme is found in the tissues of most living things and catalyses the breakdown of hydrogen peroxide into water and oxygen. (Hydrogen peroxide is a toxic product of several different metabolic reactions). I will be measuring the amount of oxygen produced when catalase reacts with hydrogen peroxide over a given time, found by my pilot test. Because gas is produced and can be collected, rate of reaction can usually be easily measured. However, as more and more substrate is converted into product, there are fewer molecules to bind with enzymes, therefore the reaction gets slower and slower. The rate of an enzyme-controlled reaction is always fastest at the beginning. This rate is called initial rate of reaction. Because I am measuring the rate of reaction over 4 minutes, this figure...

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