To determine if the rate of reaction of free enzymes is higher that the rate of reaction in immobilised enzymes in different pH concentrations of solution.

... AS Level Human Biology Investigation into the effects of pH on free and immobilised amylase By: Laura Carolan-Munslow Aim: To determine if the rate of reaction of free enzymes is higher that the rate of reaction in immobilised enzymes in different pH concentrations of solution. Introduction: Enzymes are biological catalysts that help biological reactions, which normally occur very slowly at biological temperatures, to occur faster. Enzymes do this by lowering the activation energy (the energy required to get the reaction started, and break some bonds initially). By lowering the activation energy, the reactions can occur much faster. Most enzymes are globular proteins, (though some are RNA enzymes). Protein enzymes have a 3D formation, which has to remain intact for the enzyme to function correctly. If this 3D shape is destroyed, or even altered, the enzyme cannot function. When this happens the enzyme is said to be denatured. An enzyme can be denatured in various ways;

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