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This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase.
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| Submitted: Wed Aug 27 2003
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ALFRED PHOA REF NO: 02651 BIOLOGY T1 PRACTICAL: PLAN THE EFFECT OF THE CONCENTRATION OF HYDROGEN PEROXIDE ON CATALASE IN CARROT CELLS AIMS: This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. PREDICTION: I predict that the higher the concentration of the substrate Hydrogen Peroxide (H2O2) the quicker the catalase will hydrolyse and break down the substrate into its simpler constituents. I predict that as the substrate concentration increases, the rate of reaction will go up at a directly proportional rate until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point Vmax is reached, then adding extra substrate will make no difference to the rate of reaction. HYPOTHESIS: The rate steadily increases when more substrate is added because more of the active sites of the enzyme are being used which results in more reactions so the required amount...
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