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The effects of Enzyme Concentration on the activity of Amylase
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The effects of Enzyme Concentration on the activity of Amylase Aim: To investigate the effect of Amylase concentration on its activity. the relative activity of Amylase is found by noting the time taken for the starch substrate to be broken down, that is, when it is no longer gives a blue-black colour when tested with iodine solution. This time is referred to as the achromatic point. Equipment: ? Amylase solution 0.1% ? Starch Solution 1.0% ? Distilled water ? Iodine in potassium iodine solution ? White tile and polythene pipette ? Graduate pipettes or syringes ? Test tubes in rack ? Beaker (used as water bath) ? Stopwatch, Thermometer ? Eye Protection Method: 1) Wear your eye protection 2) Prepare five different concentrations of the enzyme solution: undiluted,3/4 diluted, 1/2 diluated, 1/4 diluted, 1/10 diluted, and a control 3) Set up water bath 40°c. 4) Pipette 5cm³ of the undiluted enzyme solution into one test tube and 5cm³ of starch solution into another test tube. Stand both tubes in the water bath and leave for several minutes to reach the temperature...
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