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The effect of temperature on the rate of an enzyme catalysed reaction
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Biology AS Skills I, A & E The effect of temperature on the rate of an enzyme catalysed reaction By Rebecca Steptoe A Table to show the rates of reaction when an enzyme-catalysed reaction was carried out at different temperatures Temperature/ ºC Time taken/Secs (to nr 30s) Time taken/Mins Rate 1 . Time /Min Room (20) 165 5.5 0.2 30 150 2.5 0.4 40 120 2 0.5 50 90 1.5 0.7 60 90 1.5 0.7 70 60 1 1 See Graph: According to the graph the optimum temperature this enzyme, trypsin, works at is 80ºC. Meaning As the graph has shown us the optimum temperature for trypsin in this experiment is apparently 80ºC, however it is known that this result is not possible since enzymes are denatured at around 40ºC. As the temperature rises the enzyme and substrate molecules move faster, collisions happen more frequently so the substrate molecules enter the active site more often. Even more important is that when they collide it is with more energy that they do so; this makes it easier for bonds to be broken so...
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