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The effect of temperature on the activity of trypsin. Aim To investigate the effect of temperature on the activity of trypsin, using protein coated photographic film. Apparatus * 4 cut photographic filmstrips * Trypsin solution (0.5%) * 3 test tubes * Test tube rack * Graduated pipette filler * Water bath * Thermometer * White tile * Stop watch Method 1. Set up 4 water baths at 30 C, 40 C, 50 C and 60 C. Put a thermometer in each water bath to check the temperature. 2. Set the graduated pipette at 5cm and pipette 5cm of trypsin solution into each of the 4 test tubes. 3. Place one test tubes in each of the different water baths set at different temperatures, but because there are only 3 test tubes you can use the same test tube for 30 C and 40 C. 4. Leave the test tubes in the water baths for several minutes to reach the temperature of the water bath. 5. Cut or obtain...

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