The effect of temperature on the activity of trypsin.
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The effect of temperature on the activity of trypsin Method: To begin the experiment safety precautions were carried out, goggles and a laboratory coat was put on. A water bath had been previously prepared for us at 30c. Three test tubes were labelled '30c casein', '30c trypsin' and '30c control'. Using a pipette, 5cm? of casein suspension was pipetted into each of the test tubes labelled '30c casein' and '30c control'. Using another pipette to avoid contamination, 5cm? of trypsin solution was pipetted in the test tube labelled '30c trypsin'. And again, using a different clean pipette, 5cm? of distilled water was added into the test tube labelled '30c control'. The three test tubes were then placed into the water bath and left for several minutes to reach the temperature of the water (we tested this by placing thermometers in each test tube, we had to use three thermometers because if one...


