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The effect of temperature on an enzyme controlled reaction Introduction For this investigation I have been asked to investigate the effect of temperature on the rate of decomposition of the substrate hydrogen peroxide when catalysed by the enzyme catalase. The substrate hydrogen peroxide is a naturally produced substance, it is generated as a toxic by-product from the liver and by cell metabolism. Hydrogen peroxide, H2O2, is also produced by white blood cells as a means of killing bacteria. If the HzO2 is not broken down and left to accumulate it can be very serious as it will intoxicate the cells. This is why an enzyme is needed to catalyse the reaction, otherwise hydrogen peroxide would decompose far too slowly, the enzyme being catalase. All biochemical reactions need enzymes to catalyse the reaction, examples of enzyme-aided reactions include all digestion and growth. If there is any chemical reaction in any living thing...

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