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The effect of temperature on Membrane Permeability It is my aim to investigate whether or not temperature, or more specifically heat, affects the permeability of a cell membrane. Background Based on the fluid mosaic theory we believe the plasma membrane is composed of a phospholipid bilayer about 7-10 nm thick. This theory also suggests that proteins in bedded in the membrane are used for transporting substances across the membrane, creating pores for substances to pass through and act as carriers. Method i. The beetroot must be cut into cylinders and also washed as to remove any anthocyanin pigment that has leaked out the cells because of the cutting, then placed in distilled water ii. Heat 200cm3 of distilled water to 85oC in a sensible sized beaker iii. Whilst the water is heating, use a syringe to place 10cm3 of distilled water into each of ten test tubes. Label the tubes 85,80,75,70,65,63,60,55,50,45 (as these will be the...

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