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The effect of pH on the activity of the enzyme, Catalase.
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The effect of pH on the activity of the enzyme, Catalase. Background Information: Enzymes are substances that act as catalysts to increase the rate of a chemical reaction. In the absence of an enzyme, biological reactions occur very slowly or not at all. Most enzymes are large protein molecules, with complex three-dimensional shapes. Since they are proteins, enzymes are sensitive to changes in temperature and pH, as this will cause the shape of the enzyme molecule. Most enzymes have a characteristic pH at which they function most effectively. Changes in pH effects the ionization of side groups in the enzymes amino acids and this causes the shape of the active site to alter. Catalase is an enzyme found in many different tissues and it catalyses the breakdown of hydrogen peroxide into water and oxygen. Hydrogen peroxide is a toxic substance formed during aerobic respiration and catalase removes it. Aim: To investigate the effect of...
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