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The aim of this investigation is to investigate the effect of different temperatures on the activity of pancreatic amylase.
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Michael Thomas Biology Coursework Planning Aim: The aim of this investigation is to investigate the effect of different temperatures on the activity of pancreatic amylase. Equipment: 3,5-dinitrosalicyic acid reagent (DNSA), Sodium Chloride, ph 6.5 Buffer Solution, Distilled water, Water baths, Test tubes, Starch, Amylase, Automated pipettes, Cuvettes, Colorimeter and Timers. Background: Enzymes are biological catalysts, which assist and speed up different chemical reactions within the body by lowering the activation energy need for a reaction. Buchner first discovered enzymes in 1900 in fermenting yeast. Enzymes are proteins, the reactions only take place in a specific part of an enzyme called the active site, the amino acids which surround the active site connect to the substrate molecule holding it in place while the reaction occurs, making the enzymes specific because other molecules do not fit in the active site. Enzymes have an optimum temperature at which they work fastest, these are normally higher temperatures, Higher...
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