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The Effect of Temperature on the Activity of Amylase.
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| Submitted: Fri Mar 26 2004
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The Effect of Temperature on the Activity of Amylase The table below shows the average time taken for the amylase to denature at the five different temperatures. Temperature of amylase and starch solution (?C) Average time taken for the amylase to denature (minutes) 25 3 30 2 45 1 60 1 75 3 The table below shows the relative rate of reaction by finding the reciprocal of the time taken to reach the achromatic point at different temperatures. Temperature of amylase and starch solution (?C) Relative rate of reaction by finding the reciprocal of the time taken to reach achromatic point to 1dp (1/2) 25 0.3 30 0.5 45 1.0 60 1.0 75 0.3 There are six factors that effect enzyme activity. They are: * Temperature * pH * Substrate concentration * Enzyme concentration * Cofactors * Inhibitors The factor we are changing is temperature. The general rule for enzyme activity is the higher the temperature, the faster the reaction. However, this only works until a certain temperature. This is known as the optimum temperature. As the temperature increases between 25?C and 45?C the...
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