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The Effect of Substrate Concentration on the Rate of Reaction with Amylase
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The Effect of Substrate Concentration on the Rate of Reaction with Amylase Hypothesis: Null: - There will be no change in the rate of reaction, even with a change in substrate concentration. Alternative: - The rate of the enzyme-catalysed reaction will occur at a faster rate with an introduction of a higher concentration of substrate, and the rate of reaction will only be bounded by the availability of active sites. Introduction: The aim of this experiment is to show that the substrate concentration affects the rate of reaction, using the enzyme amylase and different concentrations of starch substrate. Similar to cellulose, starch molecules are glucose polymers linked together by the alpha-1, 4 and alpha-1, 6 glycosidic bonds, as opposed to the beta-1, 4 glycosidic bonds for cellulose. Starch is composed of two polymers, the linear amylose and the highly branched amylopectin. In order to make use of the carbon and energy stored in starch, the human...
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