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The Effect of Substrate Concentration on Enzyme Action.
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The Effect of Substrate Concentration on Enzyme Action Aim The aim of this investigation is to determine the effect of substrate concentration on enzyme action. The reaction analysed was that between the substrate hydrogen peroxide and the enzyme catalase found in yeast to break down the hydrogen peroxide. Background Catalase behaves as a catalyst for the conversion of hydrogen peroxide into water and oxygen. Catalase is an example of a particularly efficient enzyme. Catalase has one of the highest turnover numbers for all known enzymes (40,000,000 molecules/second). This high rate shows an importance for the enzymes capability for detoxifying hydrogen peroxide and preventing the formation of carbon dioxide bubbles in the blood and this is why this enzyme is formed in the body. Catalase is made in the liver when found in the body. But for the purpose of this experiment yeast shall be used as the form of catalase. (1) Hydrogen peroxide is a by-product...
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