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The Effect of Enzyme Activity An enzyme is an organic molecule which acts as a catalytic agent to speed up or facilitate biochemical reactions. Enzymes are very specific. They work by using a 'lock and key' system. The active site, of the enzyme, acts like a lock and the substrate acts like the key. The substrate in this experiment is milk. When the 'lock and key' fit together, the substrate breaks down and forms an enzyme-substrate complex. The time in which this takes is milli-seconds. The 'Lock and Key' System Trypsin us one of the three principal digestive proteases, the other two being pepsin and chymotrypsin. Trypsin is an endopeptidase, it cuts proteins in the middle of the chain. Proteins are made up of various amino acids which have different properties and make up a chain. Trypsin digests proteins at specific points. It requires the remains prior to the cut site to be positively charged. Many...

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