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Rate of enzymes reaction and the concentration of substrate
Member rating: No Rating | Words: | Submitted: Thu Jul 11 2002
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Rate of enzymes reaction and the concentration of substrate Aim to investigate the effect of substrate concentration on rate of enzyme reaction, using immobilised catalase. Hypothesis the rate of reaction will be increasing until the limited amount of enzymes. After the point of limit, the reaction rate will be stop increasing and stay at the certain point. Apparatus Syringes Various sized beakers Stop clock Manometer Distilled water Tweezers 6 boiling tubes Pieces of liver Homogeniser Buffer solution (PH7) Test tube rack Sodium alginate solution (2%) Blender Calcium chloride solution (1.5%) Hydrogen peroxide (20 volume) Ruler Glass rods Marker Risk assessment : 1. A hot water bath was used, special attention should be paid. 2. hydrogen peroxide solution was used, it should be handled with care as it is irritant. 3. Safety glasses should be worn for eye protection. 4. Enzymes are biologically active proteins, it should be handled with care. We should avoid direct contact or inhalation. 5. If we spill any peroxide solution or enzyme, spillages should be cleaned up immediately....
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