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Planning an experiment to investigate the effect of an enzyme on the rate of hydrogen peroxide breakdown.
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Planning an experiment to investigate the effect of an enzyme on the rate of hydrogen peroxide breakdown Aim To investigate the effect of celery extract concentration on the rate of hydrogen peroxide breakdown. Question: What is the most efficient concentration of celery extract enzyme to breakdown the solution of hydrogen peroxide into water and oxygen? Apparatus Hydrogen peroxide solution Celery extract solution Stand Clamp & boss 6 Test tubes Stopwatch Test tube rack 150cm conical flask + bung + tubing + 3 way tap Tubing Through Gas syringe Balance Goggles Labels Hydrogen peroxide Hydrogen peroxide will breakdown to oxygen and water in the presence of catalase. The reaction will increase with increasing enzyme concentration when molecules of hydrogen peroxide are freely available. However, when molecules of the substrate are in short supply, the increase in rate of reaction is limited and will have little effect. Celery extract (catalase) This important enzyme in present in all aerobic tissues (Vines and Rees, 1969). Since hydrogen peroxide is toxic to protoplasm it must be...
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