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Nobody had ever seen microbes or even thought they might exist because microbial effects could be explained in other ways.  

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Microbes. Nobody had ever seen microbes or even thought they might exist because microbial effects could be explained in other ways. What we now call biotechnology probably began as a kind of magic 1000's of years ago. It took a huge leap into turning into a science in the 1670s when a Dutch microscope maker called Anton van Leeuwenhoek looked at a sample of his own saliva. Factors that affect the growth of microbes are called limiting factors and influence the growth of cells in culture: - nutrient availability, temperature, pH, oxygen and build up of toxic material. An unfavourable level of any one of these factors can slow them to slow down or stop growth. Commercial Biotechnology. The commercial production of microorganisms used to be by batch culture - a culture was set up and after a period of growth it was harvested. A more recent method that has been used...

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