Investigation to find how varying substrate concentration affects the rate of hydrogen peroxide breakdown by catalase

... INVESTIGATION TO FIND HOW VARYING SUBSTRATE CONCENTRATION AFFECTS THE RATE OF HYDROGEN PEROXIDE BREAKDOWN BY CATALASE Aim I am aiming to measure the effect of varying substrate concentration on the rate of H2O2 breakdown by the enzyme catalase. Prediction I predict that when the enzyme concentration is kept constant, increasing the concentration of H2O2 will cause an increase in the reaction rate of the breakdown of the H2O2. Explanation Of Prediction Catalase is the enzyme produced in cells to prevent the accumulation of hydrogen peroxide, which is toxic. In this experiment homogenised kidney is used as a source of catalase enzymes. The catalase breaks down hydrogen peroxide into water and oxygen. 2 H2O2 --> 2 H2O + O2 The rate of production of oxygen can be measured, and gives a measure of the rate of reaction. Differing concentrations of substrate (hydrogen peroxide) will be added to a constant concentration of catalase and the rate of production of oxygen will

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