Investigating the effect of temperature on the activity of free and immobilised enzymes.
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Have a little read: ... Investigating the effect of temperature on the activity of free and immobilised enzymes Aim To investigate how temperature affects the rate of reaction of the enzyme lactase in free and immobilized states. I will investigate this using immobilised enzyme beads and free enzyme solution. Scientific background Enzymes are biological catalysts which increase the rate of reactions by lowering the activation energy needed for the reaction to tale place. The activation energy is the amount of energy needed for molecules to react when they collide. Molecules need to collide in order to react, this is known as the collision theory. When they collide they may not react, as a certain amount of energy is required to break bonds, this energy is the activation energy. Enzymes are made of a long amino acid chain; within this some molecules are attracted to each other, so the chain folds in on itself to form a 3D shape. An area
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