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Investigating the effect of temperature on the activity of protease enzyme Introduction The protease enzyme breaks down milk, which is insoluble and cloudy into amino acids, which are soluble and clear. In this investigation I will examine the effect that the variable of temperature has on the activity of the protease enzyme. Prediction I predict that as the operating temperature of the enzyme increases, the enzymes activity will increase at quite a fast rate from 0°C to around 30°C, and then continue to rise, at a much slower rate, until around 60ºC. The optimum temperature of the enzyme therefore will be approximately 60ºC. I predict that after 60° the activity of the enzyme will fall sharply. The reason for my prediction is that up to around 60°C the activity of the enzyme should increase because the heat gives the enzyme more energy meaning it is more active, however after 60°C the heat causes the enzymes...

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