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Investigating the effect of pH on the activity of an enzyme.
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Saira Hamid AS Biology ByB3(b) coursework. Investigating the effect of pH on the activity of an enzyme. Planning. In this investigation the effect of pH on the activity of protease on exposed photographic film is to be tested. In order to do this the colour change of the test solution will have to be measured, this can be done by measuring the absorbency by using the colorimeter. The results obtained will be from 0.00 being the lowest value; there is no fixed highest value, as this depends on the colour change of the test solution. The experiment will be carried out by reacting the enzyme with the substrate. The enzyme is the protease which is mixed with the pH buffer solution; the substrate is the exposed photographic film. No reaction occurs unless the substrate is present, this will be placed in a water bath and removed after a certain amount of time and colour change...
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