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Investigating how enzyme concentration affects the rate of an enzyme reaction.
Member rating: No Rating | Words: | Submitted: Fri Dec 12 2003
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Investigating how enzyme concentration affects the rate of an enzyme reaction. Introduction Hydrogen peroxide will breakdown into its products, oxygen and water, in the presence of Catalase. The rate of the reaction will increase with increasing enzyme concentration when molecules of hydrogen peroxide are freely available. However, when molecules of the substrate are in short supply, the increase in rate of reaction is limited and will have little effect. Hypothesis - Provided there is an excess substrate, an increase in enzyme concentration will lead to a corresponding increase in rate of reaction. Apparatus * Delivery Tube * Bungs * Test tubes * Test tube rack * Hydrogen peroxide * Catalase * Stop clock * pipettes Method 1. I will fill a 25cm3 measuring cylinder to the brim with water. I will then fill a 1000cm3 beaker with water. Taking care to keep the measuring cylinder full of water, I will invert it and place it in the beaker so that the mouth of...
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