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Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of Hydrogen Peroxide By Catalase In Immobilised Yeast  

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M1-Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of Hydrogen Peroxide By Catalase In Immobilised Yeast Problem: Hydrogen peroxide is a waste product of the metabolism (chemical reactions of living cells). It is a powerful oxidising agent, which would damage living cells unless it was destroyed. Hydrogen peroxide will eventually decompose into un-harmful substances such as water and oxygen however in this slow process. 2H O 2H O + O However the human body has a process which speeds up the breakdown of hydrogen peroxide. The human body produces an enzyme to speed up the breakdown of hydrogen peroxide; this enzyme is called catalase. Hypothesis: In my investigation I aim to explore one of the factors that would affect the catalysis of an enzyme; my research from textbooks, CD-ROMs (Encarta) and the Internet (www.britannica.com) tells me that factors such as; Temperature, pH, Substrate Concentration, Inhibition, Enzymes Cofactors and Enzyme concentration...

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