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An investigation into the effect of pH on an enzyme controlled reaction Aim: To investigate the influence of pH on the activity of potato catalase hydrolysing hydrogen peroxide. Background: Enzymes are biological catalysts which speed up chemical reactions but do not get involved in the chemical reaction, they remain unchanged. Enzymes are large and complex molecules that are synthesised by the cell to perform a specific function. These biological catalysts are very important because they speed up the rate of reaction at which they catalyse, otherwise the chemical reactions that take place in all living organisms would be too slow to support them. The enzyme catalase is present in cells of plants, animals and aerobic bacteria. It hydrolyses hydrogen peroxide (a harmful oxidizing agent) into water and oxygen: 2H O 2H O + O Enzymes have a three-dimensional shape due to the way the amino acid chain that makes up the chain is folded. This...

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