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Investigate the effect of temperature on enzyme (amylase) activity.
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| Submitted: Mon Mar 01 2004
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Investigating Enzymes Aim: - To investigate the effect of temperature on enzyme (amylase) activity. Introduction & Background Information As mentioned above, the aim of this experiment is to determine whether temperature has an effect on enzyme activity or not. To test this starch will be hydrolysed in to maltose with the enzyme Amylase. Fearons reagent will then be added to determine the colour and thus the amount of maltose present. This reagent will indicate the relative amounts of maltose produced at each temperature by colour comparison. The colour intensity's will then be examined by a calorimeter to provide us with the amount of absorbance. Starch Maltose Add Fearons Agent (Turns Red) Intensity examined by a Calorimeter The following shows how enzymes work : - Hypothesis: - Enzymes work best at an optimum temperature, which is 37.5oc in the human body. An increase in temperature supplies more kinetic energy to the molecules....
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