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Investigate the effect of pH on the rate of enzyme-controlled reactions.
Member rating: No Rating | Words: | Submitted: Wed Feb 11 2004
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PLAN: Investigate the effect of pH on the rate of enzyme-controlled reactions Aim: The specific enzyme that will be examined and utilized within this investigation is 'catalase' (present within a quantity of potato extract). In order to determine the enzyme's optimum pH a series of experiments must be carried out where the pH concentrations (buffer solutions) are changed, while the substrate (hydrogen peroxide) and enzyme concentrations remain constant. Hypothesis, Prediction and Scientific Background: Catalase: an enzyme, found in peroxisomes, which catalyses the decomposition of hydrogen peroxide, which results from oxidation reactions in the cell; to yield water and oxygen. High concentrations of catalase are found in the liver. Furthermore catalase is the fastest known enzyme and an important part of the body's antioxidant defences. Therefore catalase is a protein that, in small amounts, speeds up the rate of a biological reaction without itself being used up in the reaction (i.e. it...
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