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In this investigation, I am aiming to investigate the effect pH has on the activity of the enzyme, Trypsin.
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| Submitted: Mon Aug 18 2003
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Aimeé Allam Biology Coursework: Trypsin Investigation Planning Section: In this investigation, I am aiming to investigate the effect pH has on the activity of the enzyme, Trypsin. Factors affecting the activity of Trypsin: -The pH This is the independent variable. I will investigate this factor. -The temperature of the surroundings. -I will control this by doing all the tests at room temperature. This way it won't affect my results. -The concentration of Trypsin. -I will control this by using a solution of Trypsin of 5% concentration for all my tests. -The concentration of Gelatin. -I will control this by using pieces of photographic film that are exactly equal in size: 1cm squared Preliminary Testing: I performed a preliminary test to see which strength of Trypsin (1%, 3%, 5%) would be the most efficient to use, given the time we are allowed to perform the experiment. Apparatus: Test tubes x3 Test tube rack Timer x3 PH 9 buffer solution Trypsin in the following strengths: 1% 3% 5% Spatula...
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