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Identifying Variables.  

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Identifying Variables Independent Dependent Control Enzyme concentration - in my experiment, I used different concentration of enzymes (1%, 0.75% 0.5% 0.25%) to see how the rate of reaction affects by the concentration of enzymes Rate of reaction - time taken milk to be cleared - temperature ( room temperature) PH - I didn't control it and it might have changed Volume of enzyme - 5cm3 Volume of substrate which is marvel casein - 5cm3 Start timing as soon as enzyme's added Shake the concentration of marvel and enzyme gently and occasionally which is not very controlled What are the reasons for controls? * We did the experiment at the same temperature of room temperature the reason that temperature affects the rate of reaction. In fact over a range or 0 - 40 degree the rate of an enzyme controlled reaction doubles for every rise of 10 degree. Because according to Collision theory, heat increases molecular motion, thus the reactants...

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