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Background research Project title: How the level of Catechol Oxidase affects the browning on the surface of fruit. In order to draw a firm conclusion from the experiment I will be carrying out I need to know exactly what is involved in the experiment e.g. the structure of the enzyme I am using. (1) The enzyme catechol oxidase (7) It is exsists in all plant enzyme and contains a dinuclear copper centre. It is also known as polyphenoloxidase. It catalyses the substrate catechol - O.quinone is then produced. O.quinine reacts with oxygen, Melenine is then formed from the o.quinine reacting with oxygen which are brown compounds are formed from this reaction. (9) Plant cells have a large, fluid filled space in the centre this is known as a vacuole. It contains cell sap - this is made up of a watery solution of sugars, salts and sometimes pigments. The substrate Catechol can be found in the...

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