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5/2/02 Experiment to investigate the effect of temperature on enzyme activity Aim The effect of temperature on enzyme activity can be tested in different ways using different enzymes. In this experiment we will be testing the effect of temperature on the activity of Rennilase in the clotting of milk. We will test the time it takes for the milk to clot with this enzyme at different temperatures. Background Information * Enzymes are biological catalysts. * The way enzymes work can be described as the lock and key theory * Enzymes are affected by concentration * Enzymes are affected by PH * Enzymes are affected by temperature 1) Enzymes are Biological Catalysts * Enzymes speed up chemical reactions without changing them (making them a catalyst as this is what a catalyst does). * Reactions that build up larger molecules are called Anabolic reactions. * Reactions that break larger molecules to smaller molecules are called catabolic reactions. 2) The way enzymes work can be...

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