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Experiment to Investigate the Activity of an Enzyme
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Experiment to Investigate the Activity of an Enzyme (To construct a Time Progress Curve for Sucrose (Intervase) Sucrose using the enzyme sucrase (invertase) can be broken down into Glucose and Fructose. The aim of this experiment was to find about the activity of enzymes through measuring the micromoles of sucrase expressed whilst the following reaction occurs:- SUCRASE SUCROSE GLUCOSE + FRUCTOSE C12H2201 H20 C6H12O6 C6H12O6 The progress of this reaction was monitored using Dinitrosaylic Acid (DNS) as the reagent reacts with the reducing sugar products. The colour of DNS changes from yellow to varying shades of red; depending on the on the reducing sugars product being found with time. The light absorption from the varying colours of solutions can then be measured calorimetrically. A standard calibration curve could then be used to give the results in units of micromoles. Method As described in schedule. Results Tube Number 1 2 3 4 5 6 7 8 Time (in minutes) 0.5 1 2 3 4 5 6 7 Concentration 0.15 0.20 0.36 0.40 0.49 0.58 0.60 0.61 Absorbance in nm* 0.248 0.333 0.611 0.675 0.85 1.126 1.176 1.226 (Green filter No. 604) Please see graph. Discussion The graph shows at...
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