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Experiment To Investigate the Plasmolysis Rate and Concentration of Sucrose Solution In the Onion Cell  

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Experiment to investigate the plasmolysis rate and concentration of sucrose solution in the onion cell Neena Kim SFC1A Date of the experiment : 1st September 2001 Objective In the onion cell, there is cell sap which has a certain concentration of materials. Depending the concentration of the outside of the cell, the cell can be plasmolysed at the different rate by osmosis. Hypothesis The cells will be plasmolysed more as the concentration increases. And incipient plasmolysis is the point when 50% of the cells are plasmolysed. Around 0.35M point, the rate of plasmolysis is the greatest. Apparatus Onion bulb 6 petri dishes 6 test tubes test tube rack wax pencil six 10cm graduated pipettes six beakers fine brush distilled water 1M sucrose solution fine forceps paster pipettes slide and cover slips microscope graph paper lazor blade tile procedure label six % of plasmolysed cells in the onion concentration of sucrose solution 1st 2nd 3rd average 0.2M 0.0 3.6 1.7 1.77 0.3M 15.8 22.0 22.1 19.97 0.35M 46.8 56.3 38.5 47.18 0.4M 69.3 54.5 57.3 60.40 0.5M 94.6 91.2 89.2 91.70 1.0M 98.7 95.1 96.0 96.67 Table of the percentage of the plasmolysed cells in the onion and the concentration of sucrose solution used Limitation factors 1....

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