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Enzyme concentrations and enzyme activity Purpose: To investigate how enzyme concentration can affect the rate of reaction, in this case the breakdown of protein by protease enzyme. The protein substrate in this reaction will be trypsin milk protein (casein). Background: Trypsin is an endopeptidase, protease enzyme that is made in the pancreas and used to digest proteins so that the body can absorb them into the blood. The pancreatic duct in people who have cyctic fibrosis frequently becomes blocked, reducing or preventing the release of pancreatic enzyme ino the small intestine. Enzymes are biological catalysts made up from protein that changes the speed of chemical reactions without themselves being changed at the end of the reaction. Enzyme action occurs at the surface of the enzyme called the "active site" which has a shape complementary to the substrate, when the enzyme and substrate collide, the substrate locks into the enzyme's active site. The active site alters its...

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