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F.6B Fiona Chan (2) Biology Laboratory Report Date: 28-10-2004 Title: Enzyme activities Hypothesis: Papaya, pineapple and kiwi fruit contain protease which will digest the milk protein, casein, in the milk agar plate and form a clear zone. The degree of clearness can be used as an indication of protease activity. No clear zone will be found if we use the boiled fruit juice in the experiment since enzyme denature in high temperature(90oC). Aim: To determine which of the juices squeezed from the fruits(papaya, pineapple and kiwi fruit) show the greatest protease activity. Principle of design: The independent variable in this experiment is papaya, pineapple and kiwi fruit. We use a ruler to measure the diameter of the clear zone which is the dependent variable in this experiment. We also do the experiment with distilled water to act as control, thus we can check whether the effects observed are due to enzymes alone. We should incubate the petri dish...

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