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Enzyme Specificity  

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21/11/02 Enzyme Specificity Dora Winstone F26 Enzymes are specific and usually only attack one bond in the substrate, and then join to the active site. This experiment investigates the specificity of enzymes, which part in a variety of similar substrates, the enzyme will bind to. This experiment uses fermentation with yeast to test this. Glucose is the only sugar that is used in respiration so by testing a group of similar monosaccharides along with glucose, the part of the sugar that is used can be defined. All of the sugars used in this experiment are monosaccharides, which means some have the same formula as glucose but not the same structure. Fermentation in this experiment uses anaerobic respiration, without oxygen. This is the reaction for anaerobic respiration : Sugar ethanol + carbon dioxide + energy C6H12O6 2C2H5OH + 2CO2 + energy As anaerobic respiration produces carbon dioxide, measuring how much is produced by each sugar can...

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