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Biochemical Simulations. Copyright (c) David A Bender, 1982 - 1997 Enzyme Assay Units of enzyme activity Enzymes are quantified in terms of their catalytic activity - the amount of substrate that is converted to product /unit time, under specified conditions. 1 unit of enzyme activity = 1 ?mol of product formed / minute A number of factors affect the activity of an enzyme, and you will investigate most of these during this exercise: The pH of the incubation The pH of the incubation medium affects the ionisation of both the substrate and the amino acid side chains in the enzyme. Obviously, this will affect both the binding of the substrate to the enzyme and also the catalytic activity of the enzyme. Any enzyme has an optimum pH at which it shows maximum activity. For some enzymes there is a broad range of pH around this optimum where activity is nearly maximum; for others there is only a narrow...

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