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Effects of Stress on Biological Membrane Abstract In this experiment, the spectrometer was used to determine the effects of osmosis, pH, and detergent on biological membrane. Small beet pieces were soaked in various solutions and the intensity of red-pigment emitted was proportional to the damage done. Solutions with a greater concentration of salt caused more damage to the beet membrane due to osmosis. Solutions with an highly acidic and highly basic pH caused the most damage to the cell. Also, pH solutions in the neutral range 6-8, had little or no effect on the cell. Detergent solutions with a small concentration of SDS, 0% to 0.2% caused the most damage. Introduction To maintain order in a cell, the membrane consists of lipids and proteins. In a plant cell, the membrane-bound vacuole, tonoplast, is large and contains water. A beet plant's tonoplast also contains a water-soluble red-pigment, betacyanin, which acts as an indicator. When the...

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