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AP Biology lab # 4 Thomas Selby Mrs. Lipinski 10/10/03 Effect of Temperature on Enzyme Activity Abstract The objective of this experiment was to determine the effect of temperature on the rate that enzymes work. The purpose was to determine whether increasing the temp made the enzymes more active, and if so, at what temperature does the activity start to decline. The experiment consisted of thirty test tubes, with 5 test tubes at each temperature. The temperatures used were 10, 20, 30, 40, 50, and 60 degrees Celsius. For each temperature there were four test tubes with a sucrose substrate, a buffer, and an enzyme, and one test tube with just sucrose substrate, a buffer, and distilled water. After the liquids were mixed and left for exactly twenty minutes, DNS was added to each test tube and then each tube was boiled for 10 minutes, and finally the test tubes were removed from...

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