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Does temperature have an effect on the activity of dehydrogenase enzymes in yeast cells?
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| Submitted: Fri Oct 24 2003
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The Effect Of Temperature On Enzyme Activity Hypothesis: Does temperature have an effect on the activity of dehydrogenase enzymes in yeast cells? Aim: The aim of this experiment is to show the relationship between temperature and the rate of activity of dehydrogenase enzymes in two different yeast types ('Bakers' and 'Brewers'). We can then compare the results of the two different types of yeast to see which type is more affected by changes in temperature. Triphenyl tetrazolium chloride (TTC) is an artificial hydrogen acceptor, or redox indicator. When oxidised, TTC is colourless, but when it is reduced, TTC will form red, insoluble compounds called formazans. This colour change therefore shows the presence of active dehydrogenase enzymes in yeast cells. The temperature of the TTC solution and yeast suspension will affect the rate at which this colour change occurs, which in turn will show how the activity of dehydrogenase enzymes in different yeast...
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