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Diffusion of the beetroot discs dye in different bile salt concentration.  

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Analysing evidence and drawing conclusions In this experiment I generated one set of data plus two additional sets were given for the measurement of light absorbance of beetroot dye using the colorimeter. The data are listed below in Table 1. The first column shows the concentration of the bile salt, the next three columns show the absorbance of the three sets of beetroot dye. The last column gives the average absorbance value for each bile salt concentration. Table 1 Concentration of bile salt (g/100cm3) Absorbance (set # 1) Absorbance (set # 2) Absorbance (set # 3) Total Average absorbance (A) 0.0 0.08 0.08 0.09 0.25 0.08 0.1 0.11 0.07 0.04 0.22 0.07 0.2 0.13 0.08 0.06 0.27 0.09 0.3 0.10 0.11 0.17 0.38 0.13 0.4 0.16 0.17 0.20 0.53 0.18 0.5 0.21 0.09 0.21 0.51 0.17 0.6 0.24 0.09 0.27 0.60 0.20 0.7 0.30 0.15 0.31 0.76 0.25 0.8 0.14 0.13 0.32 0.59 0.20 0.9 0.25 0.25 0.36 0.86 0.29 1.0 0.32 0.25 0.41 0.98 0.32 From the above table there is a clear correlation between the concentration and the absorbance of light. As the concentration increases so does the absorbance increase i.e. the concentration is directly proportional to light absorbance. Bile salts cause beetroot cell membranes to become more permeable, therefore allowing the beetroot anthocyanin to diffuse from within the cell to the outside...

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