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Determine the extent to which a pH affects enzyme effectiveness.
Member rating: No Rating | Words: | Submitted: Fri Mar 31 2006
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The aim of this investigation is to determine the extent to which a pH affects enzyme effectiveness. This was measured via absorbency techniques, using the colorimeter. Apparatus Water Bath set to 40ºC Colorimeter Cuvettes Test tubes 10ml measuring cylinders Glass pipette Small and large beaker Tweezers Test tube rack Thermometer Live enzyme (pepsin 2%) Denatured enzyme Buffers, 2, 3, 4, 5, 6, 7, 8 Stop watch Marker pen Strips of 35mm x 5mm photographic film Sticky Labels Safety * Wear lab coat * Wash your hands thoroughly before and after the experiment. * Never eat or drink in the lab * Report any accidents, breakages or spillages * Wear goggles throughout the experiment and take precaution when handling the acidic buffers. Method * Two sets of test tubes were labelled 2, 3, 4, 5, 6, 7, 8 respectively1. * All the test tubes were placed in the rack, ready for solutions to be added to them. * Using a 10ml measuring cylinder 3ml of pepsin and 3ml of pH 2 buffer were accurately measured using separate cylinders2. *...
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